FIG. 2.

Nanog expression during zebrafish embryogenesis. (A) Temporal expression of D.r. Nanog analyzed by real-time RT-PCR. Histone H4 is used as internal control, and expression is given in relative units. (B) Expression analysis of D.r. Nanog by whole mount in situ hybridization. Four-cell to 75% epiboly are viewed from top, the insert shows a lateral view of the shield (red arrowhead). About 90% epiboly to long pec stage are shown laterally. RT-PCR, reverse transcriptase–polymerase chain reaction.