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. 2011 Oct 3;21(8):1225–1238. doi: 10.1089/scd.2011.0285

FIG. 4.

FIG. 4.

Comparison of Xvent and D.r. Nanog overexpression in Xenopus laevis. (A) Overexpression of Nanog or Xvent2 in X. laevis embryos results in similar phenotypes. Embryos are shown at stage 11 (vegetal view), at stage 18 (dorsal view), and at stage 29 (lateral view). RNAs were bilaterally injected at indicated amounts into 2-cell stage embryos or into the 2 dorsal cells at 4-cell stage, respectively. (B) Schematic presentation of real-time RT-PCR data derived from overexpression of Nanog and Xvent1/2 in X. laevis at stages 10, 11, and 12. M.m. Nanog (400 pg), D.r. Nanog (400 pg), Xvent1 (350 pg), or Xvent2 RNA (350 pg) were bilaterally injected into 2-cell stage embryos. Histone H4 was used as internal control. Green: upregulation (≥1.5), red: decrease (≤0.75), gray: no change in transcript level (0.75<×<1.5). Marker genes that were shown to be regulated by Nanog in ES cells [56] are indicated by asterisks. ES, embryonic stem.