FIG. 5.
Protein and DNA binding properties of Nanog. (A) Scheme of Nanog deletion mutants used for pulldown analysis of GST-Nanog. White box: HD; ΔHD: deletion of the HD; HD+: HD of Nanog and surrounding 5 amino acids. Note that dimerization of D.r. Nanog does not require the C-terminus. (B) Oct25 interacts with mouse as well as with D.r. Nanog. 35S methionine-labeled mouse and zebrafish Nanog proteins were incubated with the GST-fusion protein of Oct25. (C) Electrophoretic mobility shift assay of a 22mer of the murine Tcf-3 promoter. D.r. and murine Nanog but not Xvent2 interact with the Nanog binding site.