FIG. 5.

At 65 days experimental neuroma in continuity (NIC) injuries (B, D, and F) were markedly different and in contrast to the crush injury (control) histology (A, C, and E). Masson's trichrome demonstrated remnants of wallerian degeneration in the crushed nerves (A), but prominent intrafascicular scarring in the NIC nerves (B). Laminin immunostains also demonstrated the distorted perineurial layer in experimental nerves (D). Aberrant extrafascicular axonal neurofilament-200 (NF-200) profiles in the NIC nerves (E) clearly contrast to the organized axonal regeneration seen after crush injuries (E). All figures are oriented proximal (left) to distal (right), with the periphery of the nerves at the bottom of the figures; the arrows point to the described pathology (scale bar=100 μm). Color image is available online at www.liebertonline.com/neu