Fig. 6.

p66Shc is involved in VEGF-induced cell migration in ECs. HUVECs transfected with control or p66Shc siRNAs were used for measurement of EC migration using modified Boyden chamber assay (A) or wound scratch assay (B) as well as EC proliferation (C). A: cell migration stimulated with VEGF for 6 h was measured using modified Boyden chamber method. Bar graph represents averaged data, expressed as cell number counted per 10 fields (x 200) and fold change over that in unstimulated cells (control). *P < 0.05. B: confluent monolayer of HUVECs were wound scratched in the presence of VEGF (20 ng/ml) to stimulate the EC migration toward the wound area. Images were captured immediately (0 h) and 24 h after the wound scratch. C: cells were cultured in 0.2% FBS containing medium with or without 20 ng/ml VEGF for 48 h and the cell number was measured. *P < 0.05, for the fold increase induced by VEGF vs. vehicle alone (n = 3).