Fig. 5.

Effect of TRPV4 small interfering (si)RNA on flow-induced dilation of coronary arterioles. A: TRPV4-specific siRNA (100 nM) markedly reduced TRPV4 protein expression in coronary arterioles compared with scrambled siRNA (100 nM). The band density of TRPV4 protein was normalized to β-actin (an internal loading control) and the scrambled siRNA. n = 3–4. *P < 0.05 vs. control. The inset shows a representative immunoblot. B: TRPV4 siRNA inhibited flow-induced dilation of coronary arterioles. Luminal flow was generated by pressure gradients of 10–100 cmH₂O. n = 5. *P < 0.05 vs. control.