Fig. 9.

Daily mRNA expression profiles of contraction-related proteins in aortas (A–K) and mesenteric arteries (L–U) isolated from diabetic db/db mice and control mice. Male 9- to 10-wk-old db/db mice (● and dotted lines) and control mice (○ and solid lines) were euthanized at ZT5, ZT11, ZT17, and ZT23. Aortas and mesenteric arteries were harvested and cleaned, and mRNAs were quantified using real-time PCR. A: RhoA. B and L: Rho kinase 1 (ROCK-1). C and M: ROCK-2. D and N: PKC-potentiated phosphatase inhibitory protein of 17 kDa (CPI-17). E and O: Ca²⁺-independent phospholipase A₂-β (iPLA₂β). F and P: calponin-1. G and Q: calponin-2. H and R: calponin-3. I and S: tropomyosin-1. J and T: tropomyosin-2. K and U: smooth muscle protein 22-α (SM22α). n = 5 for each mouse strain at each time point. *P < 0.05; **P < 0.01, and ***P < 0.001 when compared between db/db mice and control mice at the same time point in the post hoc analysis.