Figure 3.

Insulin and BLX-1002 stimulate HCAEC proliferation in high glucose. HCAECs were incubated at 11 mM of glucose in medium supplemented with 0.5% FBS for 24 h in the presence or absence of insulin lispro (100 pM) or BLX-1002 (1 μM). Eight hours prior to the end of the incubation, cells were pulsed with [³H]thymidine. Cells were then harvested and [³H]thymidine incorporation into DNA was measured using a microplate scintillation & luminescence counter and the protein concentration of the samples was measured using DC™ Protein Assay. * denotes P < 0.05, ** denotes P < 0.01, *** denotes P < 0.001 for a chance difference vs. control by Student's t-test.