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. 2012 Apr 12;19(1):42. doi: 10.1186/1423-0127-19-42

Figure 5.

Figure 5

Shikonin pre-treatment increases level of dendritic-cell migration from skin (immunization site) to draining lymph nodes in hgp100-immunized mice. (a) Scheme for testing DC migration from skin to lymph nodes (LNs). (b) Flow cytometry analysis of the the effects of shikonin and transgenic RANTES on DC trafficking in skin. The data represent percentage of control FITC+MHC class II+ cells [in CD11c+ DCs]. (c) Schema for pretreatment of skin with shikonin or transgenic RANTES and subsequent treatment with hgp100 cDNA vaccination. (d) Flow cytometry analysis of DC-trafficking activities in test mice. Mouse abdominal immunization skin tissue sites were pre-treated with shikonin (100 μg/site/mouse) or transfected with RANTES cDNA 24 hours before immunization via gene gun bombardment for transgenic hgp100 expression. (e) Flow cytometric analysis of skin DC trafficking activity shown in (d), data represent percentage of control FITC+MHC class II+ cells [in CD11c+ DCs]. Error bars indicate SEM. (three mice per group).