Figure 5. Loss of mir-52 does not enhance the ability of ectopically-expressed lsy-6 to regulate its target, cog-1.

(A–E) Effect of mir-52 on lsy-6 mediated regulation of cog-1::gfp::cog-1 expression. Representative fluorescent image of cog-1::gfp::cog-1 transgene expression in (A) wild type worms and (B) worms with cog-1::lsy-6 transgene with corresponding DIC images (C and D, respectively). White triangles point to uterine cells. Bars represent 10 µm. (E) Percentage of worms of given genotype with cog-1::gfp expression in either uterine cell, n≥20 (range 20–68). Worms were scored in mid-to-late L4 stage.