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. 2012 May 16;7(5):e37185. doi: 10.1371/journal.pone.0037185

Table 1. Genetic interactions of the mir-51 family with retarded developmental timing mutants.

Alae at L4 to Adult transition Lethality
Straina seam cellsb complete gapped none n % burst % bag of worms n
RG733 wild type 16.0 100 0 0 20 0 0 208
RF481 wild type 16.1 100 0 0 20 0 0 109
RF491 mir-51 16.2 100 0 0 20 0 0 151
RF499 mir-52 15.9 100 0 0 20 0 0 181
RF483 mir-53 16.1 100 0 0 20 0 0 176
RF399 mir-54/55/56 16.1 99 1 0 98 0 0 228
RF692 mir-52/53/54/55/56 –c 100 0 0 16 – – –
MT7626 let-7ts @25°C – 0 50d 50 16 100 – 103
RF447 mir-51; let-7ts @25° – 0 80d 20 20 100 - 119
RF448 mir-52; let-7ts @25° – 7 73d 20 15 96 – 114
RF449 mir-53; let-7ts @25° – 0 53d 47 17 99 1 92
RF442 mir-54/55/56; let-7ts @25° – 7 21d 71 14 99 1 91
RF554 mir-48/84/241 22.6 0 100 0 40 56 37 111
RF556 mir-52; mir-48/84/241 17.7e 49f 51 0 39 3f 77 90
RF553 mir-48/84/241 22.7 0 100 0 37 66 26 125
RF555 mir-51; mir-48/84/241 21.8 0 100 0 37 42g 41 112
RF557 mir-53; mir-48/84/241 22.2 0 100 0 38 49g 39 134
RF558 mir-54/55/56; mir-48/84/241 20.6h 21g 79 0 38 25g 57 141
VT1064 mir-48/84 – – – – – 0 69 236
RF451 mir-51; mir-48/84 – – – – – 0 30i 101
RF469 mir-52; mir-48/84 – – – – – 0 5i 148
RF454 mir-53; mir-48/84 – – – – – 0 62 106
RF415 mir-54/55/56; mir- 48/84 – – – – – 0 2i 93
RF619 mir-48/241 19.1 5 95 0 21 31 49 144
RF730 mir-48/241; mjEx160[mir-54/55/56] 22.1j 9 91 0 32 66k 24k 136k
RF568 lin-46 @15° 19.4 5 95 0 40 – – –
RF569 mir-52; lin-46 @15° 17.8l 23m 77 0 39 – – –
VC894 puf-9 – 29 71 0 34 – – –
RF578 mir-52; puf-9 – 34 66 0 50 – – –
RF620 mir-52; mir-48/241 16.6n 85 15 0 20 – – –
RF625 mir-48/241; puf-9 19.2 0 100 0 19 – – –
RF626 mir-52; mir-48/241; puf-9 16.2o 0 100 0 17 – – –
a

Full genotype information can be found in Table S1.

b

seam cells counted in L4-stage worms using wIs78 or wIs79[scm::gfp], n≥18 (range 19–30).

c

indicates results not determined.

d

alae scored categorized as partially or faintly visible rather than gapped as elsewhere.

e

indicates significant difference compared to RF554 mir-48/84/241 (student's t-test, p<0.05), which contained wIs79.

f

indicates significant difference compared to RF554 mir-48/84/241 (χ2, p<0.05) which contained wIs79.

g

indicates significant difference compared to RF553 mir-48/84/241 (χ2, p<0.05) which contained wIs78.

h

indicates significant difference compared to RF553 mir-48/84/241 (student's t-test, p<0.05), which contained wIs78.

i

indicates significant difference compared to VT1064 mir-48/84 (χ2, p<0.05).

j

indicates significant difference comparing worms from the same strain, but +/− for mjEx160[mir-54/55/56] (student's t-test, p<0.05).

k

population scored for lethality is a mix of worms +/− for mjEx160[mir-54/55/56].

l

indicates significant difference compared to RF568 lin-46 (student's t-test, p<0.05).

m

indicates significant difference compared to RF568 lin-46 (χ2, p<0.05).

n

indicates significant difference compared to RF619 mir-48/241 (student's t-test, p<0.05).

o

indicates significant difference compared to RF625 mir-48/241; puf-9 (student's t-test, p<0.05).