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. 2012 May 16;7(5):e37086. doi: 10.1371/journal.pone.0037086

Figure 2. 5-aza-2′-deoxycytidine (5Aza-dC) treatment releases gene silencing in the rpt2a mutant.

Figure 2

(A) Relative luminescence intensity of WT and the rpt2a-2 mutant treated with 50 µM 5-aza-2′-deoxycytidine (5Aza-dC). Seedlings grown in MS medium for 2 weeks are transferred to MS liquid medium containing 50 µM 5Aza-dC for one week. 35S::LUC2 in WT without 5Aza-dC treatment is set as 100%. *t-test P<0.05, error bar = S.D., n = 15. (B) Relative luminescence intensity of WT and the rpt2a-2 mutant treated with 0.1 µM TrichostatinA (TSA). Seedlings grown in MS medium for 2 weeks are transferred to MS liquid medium containing 0.1 µM TSA for one week. 35S::LUC2 in WT without TSA treatment is set as 100%. *t-test P<0.05, error bar = S.D., n = 15.