Table 1. Phenotypic and primary molecular screening of the transgenic sweet potato lines from transformed cultivar Sushu-2.
Line | Rooting test* | Shoot phenotype | PCR | GUS staining | Insertion number |
OE1 | Rooted | normal | positive | stained | 1 |
OE2 | Rooted | normal | positive | stained | 1 |
OE3 | Rooted | normal | positive | stained | 1 |
OE4 | Rooted | normal | positive | stained | 2 |
OE5 | Rooted | normal | positive | stained | 1 |
OE6 | Rooted | normal | positive | stained | 1 |
OE7 | Rooted | normal | positive | stained | 2 |
OE8 | Rooted | normal | positive | stained | 3 |
OE9 | Rooted | normal | positive | stained | 2 |
OE10 | Rooted | dwarf | NT** | NT | NT |
Sushu-2 | - | normal | - | Non-stained | - |
Notes:
A rooting test was performed by culturing shoots on basic medium supplemented with 10 mg l−1 hygromycin and recorded after two weeks;
Not tested.