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. 2012 May 16;7(5):e37424. doi: 10.1371/journal.pone.0037424

Figure 4. THU (Tetrahydrouridine) influences the cell cycle in MIAPaCa-2, H441 and H1299.

Figure 4

A. After 3 days of THU exposure, the cell cycle was analyzed using flow cytometry. In accordance with cells whose growth was inhibited by THU, MIAPaCa-2, H441, and H1229 cell lines exhibited an increased rate of the G1-phase and a decrease in the S-phase. B. Cells were processed for immuno-cytochemistry with an antibody against Ki-67. Microscopic images of Ki-67-stained cells are shown in THU-treated cells and non-treated cells. Nuclei were stained with DAB. Data represents the mean ± S.D. of 3 independent experiments and significance values relate to comparisons between medium and THU treated cells.