|
Construction of KO-mutants
|
|
|
B. cereus
ATCC14579
|
|
| AV19_upBC4707_Xba_F_II |
CTATCTAGAATTTCGGCCTATCTGGACCT
|
| AV20_upBC4707_SalI_R_II |
ATAGTCGACCATTTGTTTTCCCCTTACCC
|
| AV9_downBC4707_SalI_F_new |
ATGTCGACTAAAAAGGATGACCATTTTGGTCATCCT
|
| AV10_downBC4707_PstI_R_new |
ATACTGCAGCCATTTGGGCCATCACCATGTAA
|
|
E. coli
DH5α
|
|
| acrAB-KO-Start |
ATGAACAAAAACAGAGGGTTTACGCCTCTGGCGGTCGTTCGTGTAGGCTGGAGCTGCTTC
|
| acrAB-KO-Stop |
TGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGCATATGAATATCCTCCTTAGT
|
|
Sequencing of pBKJ236Δ
bc
4707
|
|
| pBKJ236_MCS_1 |
ACACTTTATGCTTCCGGCTC
|
| pBKJ236_MCS_2 |
CAGTCTATCCCCTGGCGAA
|
|
Sequencing of KO-mutants
|
|
|
Bacillus cereus Δbc4707
|
|
| AV55_forwKO4707_1CO |
GTGACCGCTCATTACAAGGTC
|
| AV59_revKO4707_2CO |
ATGTTGTAACGCCGAGTAAAGG
|
|
E. coli
Dh5α
ΔacrAB
|
|
| acrAB-KO-control-for |
CATCGAGGATGTGTTGGC
|
| acrAB-KO-control-rev |
TTTGGGTGAGTATTCATCCAT
|
|
Construction of pBC4707
|
|
| Bc4707-Forward_EcoRI |
CCGGAATTCGCATATGAGAAAAAAAGTCATGATGTCTTTAATGTT
|
| Bc4707-Reverse_PstI |
AAACTGCAGCTTGTTGCTCACTTGTTTGTGTAGCTGGCTTTAATA
|
|
Sequencing of pBC4707
|
|
| pTTQ18-MCS-start |
TTGCGCCGACATCATAACG
|
| pTTQ18-MCS-stop |
CCTCTTCGCTATTACGCCA
|
