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. 2012 May 16;7(5):e37255. doi: 10.1371/journal.pone.0037255

Figure 1. Minigene splicing assay of BRCA1 exon 11.

Figure 1

A. The pB1 wild type (WT) version of the minigene is shown. PCMV = promoter of the pCDNA3 vector. ATG = start codon. TAG = stop codon. +3C = insertion of cytosine as the third nucleotide in exon 8. pA = poly A signal. 1 = exon 1 of the alfa globin gene. BRCA1 exons from 8 to 12 are numbered. The black solid line represents introns. Dotted lines show alternative splicing of exon 11. B. The three splicing isoforms FL, D11q and D11 and the position of specific oligos used for detection, are shown. C. Detection of BRCA1 exon 11 splicing isoforms for: _MCF7 endogenous BRCA1 (endogenous WT). _pB1 WT minigene transfected in MCF7 (pB1 WT). _pB1 minigene carrying the c.696G>A nucleotide substitution transfected in MCF7 (pB1 c.696G>A).