Table 2.
Induction of Sox9 in explanted tissues.
| Exp # |
Explants | Explant stage |
Embryonic day |
# with expression |
% with expression |
Marker | Stage harvested |
|---|---|---|---|---|---|---|---|
| 8 | Mesenchyme + PE + SU5402 bead |
HH14 | E2 | 2/24 | 8.3% | Sox9 | + 24 hrs |
| Mesenchyme + PE + PBS bead |
HH14 | E2 | 10/12 | 83.3% | Sox9 | + 24 hrs | |
| 9 | Mesenchyme + FGF8 bead |
HH14 | E2 | 5/5 | 100% | Sox9 | + 24 hrs |
| Mesenchyme + FGF19 bead |
HH14 | E2 | 6/8 | 75% | Sox9 | + 24 hrs | |
| Mesenchyme + PBS bead |
HH14 | E2 | 1/7 | 14.3% | Sox9 | + 24 hrs |
Abbreviations: PBS, phosphate buffered saline; PE, pharyngeal endoderm.