Figure 7.

Endogenous FOXO1 and FOXO3a are involved in the downregulation of mitochondrial genes in hypoxic cells. (a) Parental RPE-hTERT cells were cultured in normoxic (20% O₂) or hypoxic (<0.5% O₂) conditions for 24 h. Expression of mitochondrial target genes was determined by qRT-PCR. (b) RPE-hTERT cells were transfected with siRNAs targeting FOXO1 or FOXO3a. At 72 h post transfection, cells were placed in normoxic or hypoxic conditions for 24 h. Expression of FOXO1 and FOXO3a was determined by qRT-PCR. (c) Expression of mitochondrial target genes in the same RNA samples as those in b was determined by qRT-PCR. All data are shown as mean±S.E.M. The symbol ‘^*' indicates statistical significance, as determined by Student's t-test (P<0.05, n=3)