Figure 3.

Cytochrome c and SMAC release from mitochondria does not require ADF/cofilin activity. (a) Mitochondrial release of cytochrome c and SMAC and mitochondrial translocation of Bax were not altered in STS-treated MEFs deficient for n-cofilin (n-Cof^−/−), ADF (ADF^−/−), or both ADF/cofilin proteins (AC). Cytosolic and mitochondrial fraction of untreated and STS-treated MEFs was performed after digitonin permeabilization. (b) Cytosolic cytochrome c levels were similar in WT- and AC-MEFs during the first hour of STS treatment. PGAM was used as a loading control