Figure 5.

Nuclear condensation upon STS exposure does not require ADF/cofilin activity. (a) Time course of morphological changes of MEFs exposed to STS was similar in control and all three mutant MEF lines. Images were acquired with differential interference contrast microscopy (DIC), and nuclear morphology was determined using fluorescence microscopy employing DNA staining with Hoechst 33342. Thick arrows indicate apoptotic MEFs and thin arrows indicate non-apoptotic MEFs. Scale bar corresponds to 30 μm in all panels. (b) STS-induced apoptosis at different time points (0–4 h) was scored according to chromatin condensation and nuclear morphology. Unaltered apoptosis rates were found in n-Cof^−/−, ADF^−/−, or AC-MEFs (quantified from six independent experiments)