Skip to main content
. 2011 Dec 16;19(6):990–1002. doi: 10.1038/cdd.2011.184

Figure 4.

Figure 4

Phosphorylation of PEA-15 at Ser-116 occurs in an Akt-dependent manner in LNCaP cells. (a) Akt silencing downregulates phospho-PEA-15 expression. LNCaP cells were treated with 100 nM Scr or siAkt for 24 h. Forty-eight hours after transfection, protein lysates were analyzed by immunoblotting with the indicated antibodies. (b) LNCaP cells were treated with various concentrations of the potent small-molecule Akt inhibitor, GSK690693C, or control (DMSO). Twenty-four hours after treatment, total amounts and phosphorylation states of the indicated proteins were analyzed by immunoblotting. (c) GSK690693C inhibits the nuclear localization of ERK. Nuclear translocation of ERK was assessed by immunofluorescence microscopy 24 h after treatment with 1 μM GSK690693C or control (DMSO). Cells were immunostained with anti-ERK (red) antibodies. DAPI (blue) nuclear counterstaining was used to define the cell nuclei. The mean fluorescence ratios FN/C are shown for ERK. Values shown represent mean±S.D. The symbol ‘*' denotes statistical significance (P<0.05)