Figure 3.

Insulin pathway mutant degenerating egg chambers have caspase and lysosome activity, whereas Pwops show weak caspase activation and acidification. All egg chambers were stained with DAPI (blue), anti-cleaved caspase-3 (green) and LT (red). (a–a″) InR³³⁹ GLC Pwop stage 6 egg chamber. (b–b″) A stage 5 Pwop from chico¹ GLCs. (c–c″) A degenerating stage 4 egg chamber from chico¹ GLCs. (d–d″) A degenerating stage 5 egg chamber from Tor^ΔP GLCs. (e–e″) Pwop stage 4 egg chamber from S6k^l−1 GLCs. Pwop and degenerating egg chamber stages are approximate due to difficulties in staging abnormal egg chambers. All images were taken at the same magnification and are projections of 15–20 images. Scale bar is 15 μm