Fig. 1.

Aged mice fed with diet containing rapamycin showed impaired glucose homeostasis. a Rapamycin-induced glucose intolerance in aged mice (p < 0.05, two-way repeated measures ANOVA, n = 47 and 40 for control and rapamycin groups, respectively). b Rapamycin-fed aged mice had a significantly higher AUC than control group (p < 0.05, Mann–Whitney U test). c More aged mice in the rapamycin-fed group showed glucose intolerance than in control group (p < 0.05, Barnard’s exact test). d Rapamycin significantly reduced plasma insulin level (p < 0.01 for 0 min and p < 0.05 for 30 min after glucose injection, Mann–Whitney U test, n = 35 and 29 for control and rapamycin groups, respectively). e Rapamycin diet did not alter insulin sensitivity in aged mice (p > 0.05, two-way repeated measures ANOVA, n = 50 and 45 for control and rapamycin group, respectively)