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. 2012 Jan 25;194(6):525–539. doi: 10.1007/s00203-012-0792-3

Table 1.

Primers used in this study

Primer Sequence
tfsA 23-1179for 5′ GGGC↓CATGGATGCGCGACCCTTTTACG 3′
tfsA 23-1179rev 5′ GGGGC↓GGCCGCTTATTTTACTACAGCTTTCACTGCATTC 3′
tfsB 28-1364for 5′ GGGGGTCTCC↓CATGGCACAAATAGCACTGGAGCAAC 3′
tfsB 28-1364rev 5′GGGGC↓GGCCGCTTACTTCACCATCGCTTTTACAGC 3′
Af6 5′ GGGCCATGGATGCGCGACCCTTTTACG 3′
Br6 5′ GGGGCGGCCGCCTTCACCATCGCTTTTACAGC 3′
TfsA_RT2_for 5′ CCTATCACCGATACGGTAAGAG 3′
TfsA_RT2_rev 5′ CCGGATTATCGGAACCTATGG 3′
TfsB_RT1_for 5′ CTGAGCAATGGCGAACAATATCGG 3′
TfsB_RT2_rev 5′ GTTGAGCACTCGGAGTCAAG 3′

Restriction enzyme recognition sites are underlined; restriction sites are indicated by an arrow