Table 1.
Primer | Sequence |
---|---|
tfsA 23-1179for | 5′ GGGC↓CATGGATGCGCGACCCTTTTACG 3′ |
tfsA 23-1179rev | 5′ GGGGC↓GGCCGCTTATTTTACTACAGCTTTCACTGCATTC 3′ |
tfsB 28-1364for | 5′ GGGGGTCTCC↓CATGGCACAAATAGCACTGGAGCAAC 3′ |
tfsB 28-1364rev | 5′GGGGC↓GGCCGCTTACTTCACCATCGCTTTTACAGC 3′ |
Af6 | 5′ GGGCCATGGATGCGCGACCCTTTTACG 3′ |
Br6 | 5′ GGGGCGGCCGCCTTCACCATCGCTTTTACAGC 3′ |
TfsA_RT2_for | 5′ CCTATCACCGATACGGTAAGAG 3′ |
TfsA_RT2_rev | 5′ CCGGATTATCGGAACCTATGG 3′ |
TfsB_RT1_for | 5′ CTGAGCAATGGCGAACAATATCGG 3′ |
TfsB_RT2_rev | 5′ GTTGAGCACTCGGAGTCAAG 3′ |
Restriction enzyme recognition sites are underlined; restriction sites are indicated by an arrow