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Real-time PCR primer sequences and products.
| Gene | Primer | Sequences (5′→3′) | Product size (bp) |
|---|---|---|---|
| β-actin | β-Actin Forward | CACCACAGCCGAGAGAGAAAT | 135 |
| β-Actin Reverse | TGACCATCAGGGAGTTCATAGC | ||
| IL-2 | IL-2 Forward | GCTAATGACTACAGCTTATGGAGCA | 138 |
| IL-2 Reverse | TGGGTCTCAGTTGGTGTGTAGAG | ||
| IL-6 | IL-6 Forward | AAATCCCTCCTCGCCAATCT | 106 |
| IL-6 Reverse | CCCTCACGGTCTTCTCCATAAA | ||
| IFN-γ | IFN-γ Forward | AAGTCATAGCGGCACATCAAAC | 132 |
| IFN-γ Reverse | CTGGAATCTCATGTCGTTCATCG |
