Table 2.
LC-MS/MS parameters (MRM transition, DP, EP, CEP, CE, and CXP) and retention times of four immunosuppressive drugs and internal standards.
| CyA | TRL | SRL | RAD | Ascomycin IS | CyD IS | |
|---|---|---|---|---|---|---|
| MRM-transition | 1202.80 → 425.20 | 821.60 → 768.50 | 931.66 → 864.50 | 975.65 → 908.50 | 809.59 → 756.40 | 1216.90 → 425.50 |
| DP (V) | 100.00 | 50.63 | 41.25 | 44.38 | 50.63 | 110.00 |
| EP (V) | 10 | 10 | 10 | 10 | 10 | 10 |
| CEP (V) | 39.85 | 34.88 | 38.52 | 39.97 | 34.49 | 47.93 |
| CE (V) | 68 | 29 | 23 | 250 | 29 | 69 |
| CXP (V) | 4 | 12 | 14 | 14 | 12 | 4 |
| Retention time (min) | 1.97 | 1.81 | 1.84 | 1.85 | 1.81 | 2.00 |
| Retention time RSD% (min) | 0.245 | 0.137 | 0.186 | 0.142 | 0.148 | 0.236 |
CyA: cyclosporin A, TRL: tacrolimus, SRL: sirolimus, RAD: everolimus, CyD: cyclosporin D, IS: internal Standard, MRM: multiple reaction monitoring, DP: declustering potential, EP: entrance potential, CEP: collision cell entrance potential, CE: collision energy, CXP: collision cell exit potential, and V: volt.