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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Vet Ophthalmol. 2011 Apr 18;14(5):304–312. doi: 10.1111/j.1463-5224.2011.00877.x

Figure 3.

Figure 3

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay demonstrating apoptotic cells in fibroblast and myofibroblast cultures treated with mitomycin C (MMC). Canine corneal fibroblasts (a, b) as well as TGFβ-induced myofibroblasts (c, d) were exposed to 0.02% MMC for 2 min. Based on six nonover-lapping fields quantification, MMC untreated controls (a, c) demonstrated 1–4 TUNEL-positive cells (red) whereas MMC-treated cultures (b, d) showed 3–8 TUNEL-positive red cells. The 0.02% MMC treatment for 2 min to canine corneal fibroblast and myofibroblast did not induce significant cell death. Arrows indicate apoptotic cells. Nuclei are stained blue with DAPI; scale bar denotes 100 µm.