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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Cancer Discov. 2012 Mar 31;2(5):458–471. doi: 10.1158/2159-8290.CD-11-0284

FIGURE 1. EGFR-knockdown induces cell death in GBM cells with EGFR EC mutations.

FIGURE 1

A. EGFR domain structure. Mutations indicated in red have been documented in glioblastoma (GBM) but not in lung cancer, while those indicated in blue are seen in non-small cell lung cancer (NSCLC) and not in GBM. Roman numerals indicate subdomains within the EC domain. B. EGFR-mutant GBM lines are sensitive to EGFR knockdown. The indicated cell lines were acutely transduced with control or two different EGFR-targeted shRNAs. The extent of EGFR knockdown was assessed by immunoblot (left panel). The effects of the hairpins on cell death was assessed by trypan blue exclusion 5 days post-infection (right panel). (NHA, normal human astrocytes). C. HER2 knockdown only induces minimal cell death in EGFR mutant SF268 GBM cells. Cells were acutely transduced with control, EGFR-targeted, or HER2-targeted shRNAs. The extent of EGFR and HER2 knockdown was evaluated by immunoblot (inset). Cell death was assessed as in B. Confer Suppl. Figure 2 for results of HER2 knockdown in EGFR mutant SKMG3 GBM cells.