Table 1. Comparison of BRITER with previously reported osteogenic BMP reporter cell Lines.
S No. | Cell Line | BMP (ng/ml) | Fold Induction (Normalized FF Luc) | Fold Induction (Relative Luc) | Time (Hrs.) | Internal Control | Normalized by | Ref. |
1 | C2C12 Cells stably transfected with BRE-FFLuc construct | 50 | 5 | N/A | 16 | None | Untreated FFLuc value | 12 |
10 | 2 | N/A | 16 | None | ||||
2 | C2C12 Cells stably transfected with BRE-FFLuc construct | 19.5 | 1 | N/A | 15 | None | Untreated FFLuc value | 14 |
3 | C2C12 Cells stably transfected with BRE-FFLuc construct | 100 | 35 | N/A | 24 | None | Untreated FFLuc value | 13 |
0.5 | 2 | N/A | 24 | None | ||||
4 | BRITER( BMP depletable osteoblast, stably transfetcted with dual Luciferase construct) | 100 | 35-50 | 45-100 | 3 | SV-40 RR-Luc | SV40-RRLuc as well as untreated FFLuc values | In this study |
10 | N/A | 17 | 3 | SV-40 RR-Luc |