Table 1.
Hydrolysis of microbicides by proteases.
| Materials tested | |||||||
|---|---|---|---|---|---|---|---|
| Protease used | Protease family1 | ll-37 | PSC-RANTES | DJ007 | LSA-5 | RC101 | GRFT |
| Pepsin | A | +3 | + | NA | + | − | − |
| Papain | C | + | + | − | + | − | − |
| Elastase | M | + | + | NA | + | − | + |
| Leucine aminopepdidase | M | + | − | NA | − | − | − |
| Pronase2 | S | + | + | + | + | + | − |
| α-chymotrypsin | S | + | + | + | + | − | − |
| Proteinase K | S | + | + | + | + | + | − |
| Endoproteinase | S | + | + | + | − | + | − |
| Lys-C | |||||||
| Trypsin | S | + | +/− | NA | + | + | − |
| Number of proteases active against AMPs | 9/9 | 8/9 | 4 of 5 | 7/9 | 4/9 | 1/9 | |
| Number active/No. tested | |||||||
1
A = aspartic acid protease, C = cysteine protease, M = metaloprotease and S = serine protease;
2
Pronase from Streptococcus greseus;
3
+ indicates the material was hydrolyzed by the indicated protease, “−” indicates no hydrolysis. NA indicates the enzyme was not active in 2% SDS containing buffer that is required to solubilize the material.