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. 2012 May 17;8(5):e1002522. doi: 10.1371/journal.pcbi.1002522

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Downes et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Steps 1–3 and step 5 are based on recommendations from Bullmore & Sporns (Nature Reviews Neuroscience, 2009). Steps 4, 6 and 7 refer to techniques specific to analysis of culture activity recorded from multi-electrode arrays (MEAs, example pictured top right). The 8×8 grid indicates the recording area of the MEA (inset: close-up of two electrodes with visible neurons in their vicinity).