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. 2012 May 17;8(5):e1002643. doi: 10.1371/journal.pgen.1002643

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Blitzblau et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Indirect immunofluorescence for Red1 (green) and DAPI staining for total DNA (blue) on spread nuclei from cells at 3 hours after inoculation into SPO for wild-type cells (H119) with and without HU, cdc6-mn cells (H154) and clb5Δ clb6Δ cells (H2017). (B) Hop1 localization analysis was performed for wild-type cells with (H4471) and without HU (H119, [67]), cdc6-mn cells (H154) and clb5Δ clb6Δ cells (H2017). The enrichment of Hop1 over input DNA is plotted for chromosome III. (C) ssDNA enrichment in dmc1Δ cells (H118, [67]), and dmc1Δ cdc6-mn cells (H1584) was plotted with respect to position on chromosome VII. (D) FACS analysis was performed for dmc1Δ (H118) and dmc1Δ cdc6-mn cells (H1584). (E) CHEF gel analysis of chromosome VIII during a meiotic time course using dmc1Δ (H118) and dmc1Δ cdc6-mn cells (H4534).