Figure 3. Insertion mutants in the c1AH10 pdl1 gene are defective in Tcd supernatant release.

Mean weight gain of cohorts of M. sexta neonates fed dilutions of washed cells or supernatants from 72 hour old cultures of E. coli containing the c1AH10 cosmid with transposon inserts in either the pdl1 gene (pdl1 KO1-mutant), white, or the pWEB cosmid vector backbone insertion (CVI-wt), shaded. Error bars represent the standard error. The more potent the toxic effect, the smaller the mean larval weight. Note the loss of toxicity at higher dilutions of the supernatants of the pdl1 knock out cosmid strain. The figures in white and black above the bars represent larval mortality numbers during the 7 day assay.