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. 2012 May 17;8(5):e1002692. doi: 10.1371/journal.ppat.1002692

Figure 6. Pdl1 exhibits some haemolytic activity.

Figure 6

A weak haemolytic phenotype displayed by the E. coli heterologous pBAD30-pdl1 based expression strain. The un-induced (A) and arabinose induced (B) expression strain on agar plates containing sheep red blood cells. Note the diffuse zone of haemolysis surrounding the induced E. coli expressing Pdl1 (arrow). (C) Haemolysis of sheep red blood cells by heterologously expressed Pdl1 in a liquid assay. Controls include, PBS alone, LB medium alone, LB supplemented with 0.2% w/v arabinose and the un-induced pBAD30-pdl1 expression strain. Results are expressed as a percentage haemolysis, where 100% corresponds to the amount of haemoglobin released by complete lysis of the red blood cells using water.