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. 2012 May 17;8(5):e1002728. doi: 10.1371/journal.pgen.1002728

Figure 5. HEY binding motifs identified in silico are bound by HEY proteins in electrophoretic mobility shift assays.

Figure 5

(A) The two top motifs for DNA binding by HEY1 identified by de-novo motif discovery using the R package motifRG, based on 200 bp sequences at the summit of the 300 highest HEY1 peaks and 300 matched control sequences with similar GC-content and TSS-distance. (B) Recombinant MBP-HEY1 protein interacts with CACGTG. Binding of the biotin labeled CACGTG probe is competed increasingly by 5-, 15- and 50-fold molar excess of unlabeled CACGTG, CACGCG and CGCGCG-probes. The latter are the least effective. Shifted oligonucleotide indicated by arrow.