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. 2012 May 17;8(5):e1002707. doi: 10.1371/journal.ppat.1002707

Figure 3. NopM inhibits mating pheromone signaling in yeast.

Figure 3

(A) Immunoblot analysis of NopM and NopM-C338A isolated from yeast strain W303-1A carrying pESC-nopM (lane NopM) and pESC-nopM(C338A) (lane C338A), respectively. Proteins from W303-1A containing the empty vector pESC-leu (lane EV) were analyzed as a control. Extracted proteins (0.5 µg) were probed using the anti-NopM antibodies. A parallel SDS-PAGE gel was stained with Coomassie Brilliant Blue R-250 (B) Halo assay with glucose (Glu) or galactose (Gal) containing plates of yeast strain W303-1A harboring the plasmids pESC-nopM, the empty vector pESC-leu or pESC-nopM(C338A), respectively. A disk impregnated with 10 µg of α-factor was added to the center of the plate. (C) Growth of strain SY2227 (STE4 expression on SD/galactose plates) transformed with pESC-nopM (NopM expression on SD/galactose plates), pESC-nopM(C338A) (NopM-C338A expression on galactose plates) or the empty vector pESC-leu. Yeast cells were spread on SD medium plates containing either 2% (w/v) glucose (Glu) or 2% (w/v) galactose (Gal) without leucine.