Table 2. Primers used for sequence analysis of splA, screening of P. larvae isolates for splA, gene knockout in P. larvae 04-309, and recombinant production of SplA.

primer name	primer sequences	used for
SplA-F1	5′-ATGAGGAATATGGGCTCCG-3′	sequencing
SplA-R1	5′-CTGTTTTTTCGTTAAGCATGGTT-3′
SplA-F2	5′-ACTATCAGCAAATCGTTATTGAAGG-3′
SplA-R2	5′-TCAACTGTTGTTGCACCGG-3′
SplA-F3	5′-TATTAAACCTGGAAAAGTAGATGTCC-3′
SplA-R3	5′-TTAAAGGTTTTTAACAAGATTACCAGC-3′
SplA-F4	5′-GGTTAGCCAATATTGAAGCTCTG-3′
SplA-R4	5′-AATCCGCAGAACCTTTAGCA-3′
SplA-F5	5′-AAGATTTAATTGAAACTCTTAATGCAG-3′
SplA-R5	5′-TCGGTACTGCAGAAAACTTTG-3′
SplA-F	5′-ATATGGGCTCCGCTATTTCC-3′	screening
SplA-R	5′-AATCCGCAGAACCTTTAGCA-3′
Slp773_NcoI-for	5′-aatcaCCATGGCGAGGAATATGGGCTCCGCTATTTCC-3′	recombinant production
Slp773_XhoI-re	5′-aatcaCTCGAGTTAAAGGTTTTTAACAAGATTACCAG-3′
IBS_splA_101	5′-AAAAAAGCTTATAATTATCCTTAATAGCCCTGGAAGTGCGCCCAGATAGGGTG-3′	splA knockout
EBS1d_splA_101	5-′CAGATTGTACAAATGTGGTGATAACAGATAAGTCCTGGAAACTAACTTACCTTTCTTTGT-3′
EBS2_splA_101	5′-TGAACGCAAGTTTCTAATTTCGGTTGCTATCCGATAGAGGAAAGTGTCT-3′