Figure 3. CCL2-mCherry uptake is not due to pinocytosis.

Selected frames from time-lapse video microscopy (Movie S4) of migrating monocytes recorded at 37°C. The micropipette was dispending 1 µM CCL2-mCherry and 1 µM CCL20-EYFP with constant backpressure. Left panels CCL2-mCherry (ex 594 nm/em 620–680 nm), middle panels CCL20-EYFP (ex 488 nm/em 500–520 nm), and right panels merged frames overlaid with the corresponding phase image. Time stamps are indicated in mm∶ss.