Figure 4. CCL2 uptake is D6-independent.

Selected frames from time-lapse video microscopy (Movie S5) of migrating monocytes recorded at 37°C. The micropipette was dispending 100 nM CCL2-mCherry and 10 µM of the D6 ligand CCL22 with constant backpressure. Left panels phase images, middle panels CCL2-mCherry (ex 594 nm/em 620–680 nm), and right overlay. Time stamps are indicated in mm∶ss.