Figure 7. Pertussis toxin does not inhibit CCL2 uptake.

Selected frames from time-lapse video microscopy (Movie S8) of migrating human monocytes recorded at 37°C. Monocytes were pretreated with 2 µg/ml Bordetella Pertussis toxin (PTX) for 2 h. The micropipette was dispending 100 nM CCL2-mCherry with constant backpressure. Left panels phase images, middle panels CCL2-mCherry (ex 594 nm/em 620–680 nm), and right overlay. Inset of bottom right panel, zoomed presentation of the same cells showing randomly localized internalized CCL2-mCherry. Time stamps are indicated in mm∶ss.