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. 2012 May 17;7(5):e37137. doi: 10.1371/journal.pone.0037137

Figure 2. LGR6 enhances Wnt/β-catenin signaling in response to RSPO1–3 and this activity is inhibited by Dkk1.

Figure 2

HEK293T cells were transiently transfected with LGR6 or vector control, plus Dkk1 expression plasmids as indicated, along with the β-catenin reporter plasmid super 8× TOPFlash (firefly luciferase) and pRL-SV40 (renilla luciferase) and then stimulated with serial dilutions of purified recombinant RSPO1–2 (A), RSPO3–4 (B) or RSPO1 (C) in the presence of Wnt3a CM. Firefly luciferase activity of each well was normalized to that of renilla luciferase activity of the same well. All error bars are SEM (n = 4).