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The modifications of all synthetic precursors of pretubulysin (I–VIII) are highlighted [I: Mep replaced by N-methylsarcosine; II: desmethyl Tup variant; III: missing propionate of Tup; IV: MepIleTuv; V: MepIleMe'Val' (γ-amino acid); VI: MepIleMeVal; VII: MepIle; VIII: Mep].
