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. 2012 May 17;7(5):e37416. doi: 10.1371/journal.pone.0037416

Figure 11. Inhibition of L3.6pl pancreatic cancer cell migration by pretubulysin and tubulysin A.

Figure 11

A) ‘Wound healing’ assay. A confluent layer of L3.6pl was scratched and either left untreated or stimulated with varying concentrations of drug (n = 2). Left panel: Regions covered with cells are shown in yellow, and uncovered areas are displayed in gray. Right panel: The percentage of migration is expressed relative to the starvation control (0%) or the untreated control containing FCS (fetal calf serum; 100%). B) Boyden chamber assay with L3.6pl cells that were left untreated or stimulated with 1 or 10 nM drug (n = 2). Cells migrating toward a FCS gradient were stained with crystal violet (left panel), and the percentage of migration (right panel) was expressed relative to the levels of migration for the negative controls with (100%) or without FCS (*p≤0.05 Anova/Dunnett).