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. 2012 May 17;7(5):e37365. doi: 10.1371/journal.pone.0037365

Figure 2. Embryos lacking both Notch2 and Notch3 have disrupted blood vessels.

Figure 2

Hematoxylin and eosin staining of transverse sections of E11.5 embryos through the heart and midsection (A–D), descending aorta (E–H), and caudal aorta (I–L). In the Notch2−/−;Notch3−/− (N2−/−;N3−/−) embryos, the paired dorsal aorta is expanded in size and filled with blood (D, arrowheads). Higher magnification of blood vessels in double mutant embryos show a lack of cells surrounding the lumen (H, L). The overall structure of blood vessels appears relatively normal in the single Notch2−/− (N2−/−;N3+/+) and Notch3−/− (N2+/+;N3−/−) mice. Scale bar = 100 µm. (H) heart, (nt) neural tube.