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. 2012 May 17;7(5):e37365. doi: 10.1371/journal.pone.0037365

Figure 6. Smooth muscle differentiation and Notch target genes are down regulated in Notch2 and Notch2/Notch3 double mutants.

Figure 6

Gene expression analysis (qPCR) using yolk sac RNA demonstrates a reduction of smooth muscle-specific genes SMA, SM22α, and Cnn1 in Notch2−/− (N2−/−;N3+/+) and Notch2−/−;Notch3−/− (N2−/−;N3−/−). Notch signaling target gene Heyl shows a robust decrease at E10.5 and E11.5 in the Notch2−/− (N2−/−;N3+/+) and Notch2−/−;Notch3−/− (N2−/−;N3−/−) yolk sacs, while Hey2 shows a slight decrease and Hes1 expression is unaffected in the mutant genotypes. Data represent relative mRNA expression levels normalized to 18S rRNA. * P<0.05, ** P<0.01 compared to wildtype.