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. 2012 May 17;7(5):e37092. doi: 10.1371/journal.pone.0037092

Figure 1. Effects of deleting the ORC-, Abf1- or Rap1-binding site from HMR-E on transcriptional silencing at HMR.

Figure 1

(A) Effects of HMR-E mutations on HMR-silencing in the presence of HMR-I silencer. The silencing reporters Tel V-R-URA3 and HMR::TRP1 are illustrated at the top. The intact and mutant HMR-E silencers are shown on the left. Serial 10 fold dilutions of two independent clones of each of strains 1 through 8 were incubated at 30°C for two overnights on synthetic complete (SC), SC + 5-fluoroorotic acid (FOA), and SC lacking tryptophan (-Trp) media. The growth phenotypes are shown on the right. (B) Effects of HMR-E mutations on HMR-silencing in the absence of HMR-I. Growth phenotypes of strains 9 through 16 on SC, FOA and -Trp media are shown on the right. Note growth phenotypes of strains 13 and 14 on –Trp medium after one overnight (1 o.n.) incubation are also shown.