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. 2012 May 17;7(5):e37092. doi: 10.1371/journal.pone.0037092

Figure 4. Protosilencers do not modulate chromatin structure in the absence of Sir proteins.

Figure 4

Top, the modified HML locus in strain 17s. The black bar indicates the sequence corresponding to the probe used in indirect end labeling. Bottom, chromatin mapping in strains 17s through 21s by MNase digestion and indirect end labeling. MNase treated chromatin in each strain was digested with SnaBI and EcoNI and fractionated on an agarose gel. After Southern-blotting, DNA fragments ending at the SnaBI site were detected by hybridization with the probe shown at the top. The positions of the HMR-E silencer and FRT site are shown on the left of the blot. M, DNA markers. N, naked genomic DNA from strain 17s treated with MNase.