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. 2012 May 17;7(5):e37345. doi: 10.1371/journal.pone.0037345

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Alvarado et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A Solanum sogarandinum cyclophilin antibody was used for detection of cyclophylin in ZC and healthy potato plants. A. Western blot analysis showing cyclophilin (17.5 kDa, indicated by arrow) detection in both stem (S) and tubers (T) of healthy (H) tissues, but no detection in tubers of Zebra complex (Z) diseased plants. B. Coomassie brilliant blue stained gel of the protein samples used for the western blot in A, the protein concentration was calculated by Bradford assay and equal amounts of proteins (7.5 ug) were loaded. Size markers are indicated.