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. 2012 May 17;7(5):e36795. doi: 10.1371/journal.pone.0036795

Figure 6. Influence of ethylene (ACC) on SlPPC2 promoter activity.

Figure 6

Fruit slices from 8 daa fruit («WVa 106» cherry) were transformed by biolistic with a 35S:GUS plasmid co-delivered with SlPPC2 promoter:LUC fusion pPPC2pro1:LUC plasmid and incubated for 20 h on CPW4 medium supplemented with 1-aminocyclopropane-1-carboxylic acid (ACC, 20 µM and 200 µM), silver thiosulfate (AgTS), or ACC (20 µM) plus AgTS or NaTS. Control was CPW4 medium. Data were normalized using the 35S:GUS construct as internal standard and are expressed as % of the control. The mean values and SE of 12 independent transformations are shown. * indicates a significant statistical difference using a Student’s t-test (*: P<0.05; **: P<0.001).