Figure 3.

The effect of PGRMC1 siRNA on the levels of PGRMC1 in hGL5 cells. hGL5 cells were cultured with either scramble (60 nM) or PGRMC1 siRNA (30 or 60 nM) for 3 days. PGRMC1 was assessed by real-time PCR (A), Western blot (B) or immunocytochemistry (C, D). Panel C show cells cultured with scramble, while cells treated with PGRMC1 siRNA are shown in panel D. Images shown in panels B, C and D were taken after treatment with 60 nM siRNA. Data from Peluso et al [26].